
Overview:
MYT1isamemberofafamilyofneuralspecific,zincfinger-containingDNA-bindingproteinswhichbindstothepromoterregionsofproteolipidproteinsofthecentralnervoussystemandplaysaroleinthedevelopingnervoussystem.MYT1oftheoligodendrocytelineage,alongwithacloselyrelatedCCHCzincfinger,isexpressedindevelopingneuronsinthemammaliancentralnervoussystem(1).TherecombinantMYT1fragmentscontainingeithertheupstream2zincfingersorthedownstream4zincfingersboundthesamecisregulatoryelementinthePLP1promoterinvitroandDNAbindingrequiredZn(2+),butnototherdivalentcations(2).
GeneAliases:
PKMYT1,FLJ20093,DKFZp547K1610
GenbankNumber:
NM_004203
References:
1.Kim,J.G.et.al:Myelintranscriptionfactor1(Myt1)oftheoligodendrocytelineage,alongwithacloselyrelatedCCHCzincfinger,isexpressedindevelopingneuronsinthemammaliancentralnervoussystem.J.Neurosci.Res.50:272-290,1997.2.Kim,J.G.et.al:Novelmemberofthezincfingersuperfamily:aC(2)-HCfingerthatrecognizesaglia-specificgene.Molec.Cell.Biol.12:5632-5639,1992.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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