
Overview:
OSR1 is a member of the Ser/Thr family of protein kinases. OSR1 is activated by osmotic stresses and regulates the downstream protein kinase such as PAK1 in order to establish the sensor/signaling modules that initiate the cellular response to environmental stress (1). OSR1 can be phosphorylated by ATM or ATR during DNA damage process (2).
Gene Aliases:
OXSR1; KIAA1101
Genbank Number:
NM_005109
References:
1. Chen W. et al: Characterization of OSR1, a member of the mammalian Ste20p/germinal center kinase subfamily. J. Biol. Chem. 279:11129-11136(2004). 2. Matsuoka S. et al: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316:1160-1166 (2007).
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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