
Overview:
PDE3A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3A has high affinity for both cAMP and cGMP and shows competitive inhibition of the cAMP hydrolytic activity by cGMP (1). Deletion of the N-terminus of PDE3A enhances the hydrolysis of cGMP relative to cAMP suggesting that the role of divergent N-termini of various PDEs could be to exert substrate specificity. Stimulation of platelets with thrombin increases PDE3A activity via Akt signaling pathway and this increase can be diminished by dephosphorylation of PDE3A by protein phosphatase 1 (2).
Gene Aliases:
CGI-PDE
Genbank Number:
NM_000921
References:
1. Meacci E, et al: Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase. Proc. Nat. Acad. Sci. 89: 3721-3725, 1992.2. Zhang W, et al: Thrombin regulates intracellular cyclic AMP concentration in human platelets through phosphorylation/activation of phosphodiesterase 3A. Blood. 2007 Sep 1;110(5):1475-82.
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

