
Overview:
PDE8B or phosphodiesterase 8B is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP (1). The recombinant PDE8B exhibits cAMP-PDE activity that is resistant to several PDE inhibitors. The cAMP hydrolytic activity of PDE8B is unaffected by cGMP and it does not display any cGMP mediated hydrolysis. Defects in PDE8B are a cause of autosomal dominant striatal degeneration (ADSD) (2). PDE8B plays a prominent role in degrading cAMP expression in human embryonic kidney cells.
Gene Aliases:
ADSD; FLJ11212; PPNAD3
Genbank Number:
NM_003719
References:
1. Hayashi, M. et.al: Genomic organization, chromosomal localization, and alternative splicing of the human phosphodiesterase 8B gene. Biochem. Biophys. Res. Commun. 297: 1253-1258, 2002. 2. Horvath, A. et.al: A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex. Europ. J. Hum. Genet. 16: 1245-1253, 2008.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

