
Overview:
RAC1isamemberoftheRhofamilyandisaGTPasethatispartofthesmallGTP-bindingproteinsuperfamily.RAC1isinvolvedinadiversearrayofcellularevents,includingthecontrolofcellgrowth,cytoskeletalreorganization,andtheactivationofproteinkinases(1).RoleofRAC1incolorectalcancerhasbeenreportedafteranalysisoftheproteinexpressionlevelandactivitiesofthisproteininmatchedsetsoftumorandnon-tumortissues.OverexpressionofRAC1leadstoincreasedtumourgrowthinxenograftsofhumancolorectaltumourcells(2).RAC1isalsoinvolvedintheregulationofcriticalcellularfunctionsincludingorganizationofactinCytoskeleton,transcriptioncontrolandcellcycle.
GeneAliases:
MIG5,TC-25,p21-Rac1,MGC111543
GenbankNumber:
NM_006908
References:
1.Takami,Y.etal:TheactivityofRhoAiscorrelatedwithlymphnodemetastasisinhumancolorectalcancer.DigDisSci.2008;53(2):467-73.2.delPulgar,T.G.etal:DifferentialexpressionofRac1identifiesitstargetgenesanditscontributiontoprogressionofcolorectalcancer.Int.J.Biochem.CellBiol.2007;39(12):2289-302.
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

