Overview:
RGS1 is a member of the regulator of G-protein signalling family and attenuates the signalling activity of G-proteins. RGS1 binds to activated GTP-bound G alpha subunit and acts as a GTPase activating protein (GAP) thereby increasing the rate of conversion of GTP to GDP and terminating the signal (1). RGS1 is extensively up-regulated in renal cell carcinoma (RCC) tissues and melanoma. In melanoma, RGS1 expression is significantly correlated with increased tumor thickness, mitotic rate and presence of vascular involvement. Furthermore, there is significant association between increasing RGS1 expression and reduced relapse-free survival as well as disease-specific survival (DSS) survival (2).
Gene Aliases:
IR20, IER1, 1R20, BL34
Genbank Number:
BC015510
References:
1. Sierra D A, et al: Evolution of the regulators of G-protein signaling multigene family in mouse and human. Genomics 79: 177-185, 2002. 2. Rangel J, et al: Novel role for RGS1 in melanoma progression. Am J Surg Pathol. 2008 Aug;32(8):1207-12.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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