
Overview:
TNFB is a member of the tumor necrosis factor cytokine family that is primarily expressed on lymphocytes that control lipid metabolism. TNFB is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF-beta acts as a novel therapeutic route for the treatment of dyslipidemia (1). The presence of TNFB accelerates the initiation of immune responses and protects the host from larger viral challenges (2).
Gene Aliases:
TNF-B; LT; TNFSF1; LTA
Genbank Number:
AAH34729.1
References:
1. Lo, J. C. et.al: Lymphotoxin beta receptor-dependent control of lipid homeostasis. Science 316: 285-288, 2007. 2. Lund, F. E. et.al: Lymphotoxin-alpha-deficient mice make delayed, but effective, T and B cell responses to influenza. J. Immun. 169: 5236-5243, 2002.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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