
Overview:
TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. TP53RK belongs to a small subfamily of atypical protein kinases. TP53RK is expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes and lower expression of TP53RK is found in heart, kidney, and spleen (1). PRPK interacts with CGI-121 and co-precipitation of p53 with PRPK is inhibited by adding recombinant CGI-121 in vitro. CGI-121 may act as an inhibitor of the PRPK-p53 interaction (2). TP53RK is activated by another kinase, Akt/PKB, which phosphorylates TP53RK at Ser250.
Gene Aliases:
BUD32; C20orf64; dJ101A2; Nori-2; Nori-2p
Genbank Number:
NM_033550
References:
1. Abe, Y.et.al: Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes. J. Biol. Chem. 276: 44003-44011, 2001. 2. Miyoshi, A.et.al: Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. Biochem. Biophys. Res. Commun. 303: 399-405, 2003.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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