
Overview:
UBE2F or ubiquitin-conjugating enzyme E2F is part of the ubiquitin network for protein degradation and is involved in protein neddylation. UBE2F has been shown to function as a NEDD8-conjugating enzyme in vitro and in vivo (1). Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates Cullin Ring ligase (CRL) ubiquitination activity and prevents binding of the inhibitor CAND1 (2).
Gene Aliases:
NCE2
Genbank Number:
NM_080678
References:
1. Huang, D T. et al: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4):483-95. 2. Boh, B K. et al: Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011 Jun 3;409(2):136-45.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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