
Overview:
UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. UBE2J1 is play an important role in the homeostatic regulation of MHC class I assembly and expression (1). HRD1 and UBE2J1 associate in a complex together with non-?2m bound MHC class I heavy chains, Derlin 1, and p97 and discriminate misfolded MHC class I from conformational MHC I-?2m-peptide heterotrimers.
Gene Aliases:
CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; Ubc6p
Genbank Number:
BC013973
References:
1. Burr ML. et. al: HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation. Proc Natl Acad Sci U S A. 2011 Feb 1.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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