
Overview:
Ubiquitin is a highly-conserved protein that is widely expressed in eukaryotes (1). Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal and normal proteins with a rapid turnover. A mutant form of ubiquitin, UBB+1,accumulates in the pathological hallmarks of tauopathies, including Alzheimer's disease, polyglutamine diseases, liver disease and muscle disease and serves as an endogenous reporter for proteasomal dysfunction in these diseases. By inhibiting the proteasome machinery, UBB+1 is pathogenic, since it promotes accumulation of cytotoxic misfolded amyloid-beta and phosphorylated-tau (2).
Gene Aliases:
UBB, MGC8385, FLJ25987
Genbank Number:
NM_018955
References:
1. Baker, R T. et al: The human ubiquitin gene family: structure of a gene and pseudogenes from the Ub B subfamily. Nucleic Acids Res.15: 443-463, 1987. 2. Fratta, P. et al: Mutant ubiquitin UBB+1 is accumulated in sporadic inclusion-body myositis muscle fibers.Neurology. 2004 Sep 28;63(6):1114-7.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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