

Highlights
- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Description
Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
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Elution Volume | ≥ 6 µl of DNA Elution Buffer |
Equipment | Microcentrifuge |
Purity | A260/A280 >1.8, A260/A230>1.8 |
Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
Size Range | 50 bp to 23 kb |
Yield | Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70% |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Zymoclean has been validated for use with Zymo-Spin IIC and IIN columns. Other columns with higher binding capacity have not been validated by Zymo Research but have successfully been used by users.
Q3: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Q4: Should more Agarose Dissolving Buffer be used for higher % gels?
Add 4 volumes of Agarose Dissolving Buffer for agarose gels >2% and ensure the gel slice has been fully dissolved.
Q5: Can Zymoclean be used for samples in solution (without agarose gel)?
No, high efficiency recovery is not guaranteed and the success of the purification can be variable.
Q6: Can Zymoclean be used to recover ssDNA?
If only isolating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Recovery Kit. With some modification of the Zymoclean Gel DNA Recovery protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Q7: What is the maximum weight of gel slice that can be used?
Up to 400 mg of agarose gel.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
-Simin H. (University of California, Irvine)
Read MoreCat # | Name | Size | Price | |
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C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 |
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

