
Catalog number | TE187S |
Product type | ELISA |
Quantity | 96 Tests (12x8 breakable strip wells) |
Standard range | 0.1-18 ng/ml |
Analytical Sensitivity | 0.1 ng/ml |
Sample volume | 25 µl/well |
Species | Human |
Storage and Stability | Product should be stored at 2-8 °C. |
Precautions | For research use only. Not for use in diagnostic procedures. |
References | 1. Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertil. Steril., 1991; 55: 1202-1203. 2. Granoff, A.B. and Abraham, G.E., Peripheral and adrenal venous levels of steroids in a patient with virilizing adrenal adenoma. Obstet. Gynecol., 1979; 53:111-115. 3. Bricaire, C., Raynaud, A., Benotmane, A., et al., Selective venous catheterization in the evaluation of hyperandrogenism. J. Endocrinol Invest., 1991; 14: 949-956. 4. Heinonen, P.K., Androgen production by epithelial ovarian tumours in post-menopausal women. Maturitas, 1991; 13: 117-117-122 5. Tietz, N.W. ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580. 6. USA Center for Disease Control/National Institute of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories"”84 7. ICN Guide to Endocrine Testing. Diagnostic Division, ICN Biomedicals, Inc. pp. 2:33-35; 3:4-6. |
Testosterone ELISA Package InsertType : PDF
Testosterone, Free ELISA

Mouse/Rat Testosterone, Total ELISA

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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

