
Product Name | SensoLyte ® Anti - PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) *Colorimetric* |
Size | 1 kit |
Catalog # | AS-55524 |
US$ | $480 |
SensoLyte® Anti-PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (139-151) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (139-151) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (139-151) peptide and pre-blocked with BSA. The amount of anti-PLP (139-151) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays. Convenient Format * Pre-coated and pre-blocked 96-well strip plate * Ready-to-use substrate solution and other assay components * 2-3 hours assay time at room temperatureMinimal Sample Size * Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assayHigh Sensitivity * Detects as low as 100 pg anti-PLP (139-151) IgG * No cross-reactivity with anti-PLP (178-191) IgGBroad Dynamic Range * 8-500 ng antibody/ml serum (depending on colorimetric developing time) | |
Detailed Information | ![]() ![]() |
Storage | 4°C |
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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