
Product Name | SensoLyte ® Anti - PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) *Colorimetric* |
Size | 1 kit |
Catalog # | AS-55524 |
US$ | $480 |
SensoLyte® Anti-PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (139-151) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (139-151) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (139-151) peptide and pre-blocked with BSA. The amount of anti-PLP (139-151) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays. Convenient Format * Pre-coated and pre-blocked 96-well strip plate * Ready-to-use substrate solution and other assay components * 2-3 hours assay time at room temperatureMinimal Sample Size * Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assayHigh Sensitivity * Detects as low as 100 pg anti-PLP (139-151) IgG * No cross-reactivity with anti-PLP (178-191) IgGBroad Dynamic Range * 8-500 ng antibody/ml serum (depending on colorimetric developing time) | |
Detailed Information | ![]() ![]() |
Storage | 4°C |
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

