
Description:
KAPAStrandedRNA-SeqKitwithRiboErase(HMR)KAPAStrandedRNA-SeqwithRiboErase
Evolvedtofocus.
TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.
- Upto99.98%rRNAdepletion*
- FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
- Robustandreproducibleacrossvariousinputamounts
- Automation-friendlyworkflow
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
IndustryleADIngrRNAdepletion
- SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
- MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
Unsurpassedsequencingdataquality
- Detectionofmoregenesanduniquetranscripts
- Accurateandclearidentificationofsplicesitesandalternativegenesplicing
- ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
Highlyreproduciblesequencingresults
- Highcorrelationevenbetweendifferenttestingconditions
- Lowsample-to-samplevariationformorereliableresults
Improvedcoverageuniformity
- Uniformdistributionofreadsovereachtranscript
- Minimal5’–3’biasacrosstranscripts
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAStrandedmRNA-SeqKits

KAPARNAHyperPrepKitswithRiboErase

KAPAmRNAHyperPrepKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits
Applications:
Applications- WholeTranscriptome
- GeneExpression
- TranscriptDiscoveryandAnnotation
- TotalRNA-Seq
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto10monthsat-20ºC.
KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).
Components
Specifications
- SpecDescription
- CompatiblePlatformIlluminaHiSeq®,NextSeq®andMiSeq®
- LibraryTypeRNA
- StartingMaterialHigh-qualityTotalRNA
- InputAmount100ng-1µg
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
向左转|向右转

