
Description:
KAPASingle-IndexedAdapterKit,SetA(30µM)KAPAmRNAHyperPrepKits
Single-DayRNA
TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.
Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:
- single-daylibraryconstruction,inclusiveofmRNAcapture
- reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
- flexibleinputof50ng–1µgtotalRNA*
- maintainover99%strandspecificity*
- KAPAPureBeadsincludedforreactionpurifications
KAPARNAHyperPrepKits
KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Sequencewhatmatters
- WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievesuperiorcoverageuniformity
- Obtainmoreuniformdistributionofreadsacrosstranscripts
- ImprovecoverageofdifficultGC-richtranscripts
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPARNAHyperPrepKitswithRiboErase

KAPAStrandedmRNA-SeqKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits
Applications:
Applications- Geneexpressionanalysis
- Detectionofgenefusions,isoforms,andotherstructuralvariants
- Noveltranscriptidentification,includingnoncodingtranscripts
- SNVdiscovery
- Wholetranscriptome(KAPARNAHyperPrepKitsonly)
- Targetedtranscriptome(KAPARNAHyperPrepKitsonly)
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageEnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)
KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.
Specifications
- SpecDescription
- CompatibilityPlatformIlluminaHiSeq,NextSeq,andMiSeq
- LibraryTypeRNA
- StartingMaterialRNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA
- StartingMaterialRNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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