
Description:
KAPASingle-IndexedAdapterKit,SetA+B(30µM)KAPARNAHyperPrepKitswithRiboErase(HMR)
Single-DayRNA
TheKAPARNAHyperPrepKitswithRiboErase(HMR)utilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualityrRNA-depletedRNA-seqlibraries.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablesefficientreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredforrRNAdepletionandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:
- single-daylibraryconstruction,inclusiveofrRNAdepletion
- reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
- flexibleinputof25ng–1µgtotalRNAfromhuman,mouse,orratspecies*
- highersuccessrateswithlowerinputanddegradedsamples
- maintainover99%strandspecificity*
- KAPAPureBeadsincludedforreactionpurifications
NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Sequencewhatmatters
- WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievesuperiorcoverageuniformity
- Obtainmoreuniformdistributionofreadsacrosstranscripts
- ImprovecoverageofdifficultGC-richtranscripts
Generatehigh-qualitylibrariesfromdegradedsamples
- Inputaslittleas25ngwithFFPEsamples,dependingontotalRNAquality
- Achievelowduplicationratesandhighlyefficient,reproduciblerRNAremovalwithdegradedsamples
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievereliableresultswithdegradedinputs
- Attainahigh-degreeofexpressioncorrelationbetweenpairedFFPEandfreshfrozensampleswhichprovidesincreasedconfidenceinsequencedataaccuracy
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAmRNAHyperPrepKits

KAPAStrandedmRNA-SeqKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits
Applications:
Applications- Wholetranscriptome
- Geneexpressionanalysis
- Detectionofgenefusions,isoforms,andotherstructuralvariants
- Noveltranscriptidentification,includingnoncodingtranscripts
- SNVdiscovery
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageEnzymesandbuffersforrRNAdepletion,CDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.KAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)
KitscontainHybridizationBuffer,HybridizationOligos(HMR),DepletionBuffer,RNaseH,DNaseBuffer,DNase,Fragment,PrimeandEluteBuffer(2X),1stStrandSynthesisBuffer,KAPAScript,2ndMarkingBuffer,2ndStrandSynthesis&A-TailingEnzymeMix,LigationBuffer,DNALigase,PEG/NaClSolution,KAPAPureBeads,LibraryAmplificationPrimerMix(10X),andKAPAHiFiHotStartReadyMix(2X).
Specifications
- SpecDescription
- CompatiblePlatform0616
- LibraryTypeRNA
- StartingMaterialHigh-qualityanddegradedtotalRNA
- InputAmount25ng–1µg
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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