- AR-42 (OSU-HDAC42)
- M344
- LAQ824 (NVP-LAQ824,Dacinostat)
- RG2833
- NCH 51
- Valproic acid
| CI994 (Tacedinaline)HDAC inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity = 98.17%
- COA (Certificate Of Analysis)
- HPLC (Retest)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
Related Biological Data
| Description | CI-994 (Tacedinaline),an anti-cancer drug, is an inhibitor of HDAC1 with IC50 of 0.57 μM and causes G1 cell cycle arrest. | |||||
| Targets | HDAC1 | |||||
| IC50 | 0.57 μM | |||||
| Cell experiment: [1] | |
Cell lines | Peripheral blood lymphocytes |
Preparation method | The solubility of this compound in DMSO is ≥50mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
Reaction Conditions | 10 µM, 24 hours |
Applications | No evidence of apoptosis or necrosis was detected in lymphocytes exposed to CI994 for 4 hours. After 24 hours, concentration-dependent increases in apoptosis characterized by DNA condensation, DNA fragmentation, and/or externalization of phosphatidyl serine were seen at CI-994 concentrations as low as 1 µM and were statistically significant beginning at 10 µM. |
| Animal experiment: [2] | |
Animal models | Male Wistar rats |
Dosage form | Oral administration, 45 mg/kg |
Applications | 15 rats per group were administered a single dose of CI994 at 0 (vehicle control), 10, 23, and 45 mg/kg. 5 rats per group were killed 1, 3, and 7 days after dosing for evaluation of blood and bone marrow lymphocyte counts. CI994 treatments resulted in significant dose-related reductions in total white blood cell counts, total lymphocytes and lymphocyte subpopulations. Relative to controls, reductions on day 1 ranged from 36 to 48% at 10 mg/kg, 65 to 76% at 23 mg/kg, and 74 to 87%at 45 mg/kg. Dose-related reductions in monocytes ranging from 54 to 89% were observed in all drug-treated groups on days 1 and 3. Besides that, significant dose-related reductions in bone marrow lymphoid cells ranged from 28 to 33% at 10 mg/kg, 63 to 69%at 23 mg/kg, and 80 to 87%at 45 mg/kg. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1] Graziano M J, Spoon T A, Cockrell E A, et al. Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline). BioMed Research International, 2001, 1(2): 52-61. [2] Graziano M J, Galati A J, Walsh K M. Immunotoxicity of the anticancer drug CI-994 in rats: effects on lymphoid tissue. Archives of toxicology, 1999, 73(3): 168-174. | |
CI994 (Tacedinaline) Dilution Calculator
calculate
CI994 (Tacedinaline) Molarity Calculator
calculate
| Cas No. | 112522-64-2 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | 4-acetamido-N-(2-aminophenyl)benzamide | ||
| Canonical SMILES | CC(=O)NC1=CC=C(C=C1)C(=O)NC2=CC=CC=C2N | ||
| Formula | C15H15N3O2 | M.Wt | 269.3 |
| Solubility | ≥50 mg/mL in DMSO, <2.41 mg/ml="" in="" etoh,=""><2.18 mg/ml="" in="" h2o=""> | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
CI994 is a selective inhibitor of HDAC1 with IC50 value of 0.57 μM [1].HDAC1 is an enzyme that encoded by HDAC1 and plays a pivotal role in regulating eukaryotic gene expression. It is also been reported that HDAC1 interacts with retinoblastoma tumor-suppressor protein which is a key element in the control of cell proliferation and differentiation. Many studies have shown that HDAC1 inhibition works as a promising therapy used for cancer treatment in clinic [2] [3].CI994 is a selective HDAC1 and HDAC3 and is different from the reported unselective inhibitor SAHA. When tested with HeLa cervical carcinoma cells, CI994 treatment showed a selectivity inhibitory ability for HDAC1 and 3 with the IC50 was 43.5±1.2 μM [4]. In peripheral blood lymphocytes isolated from untreated male Wistar rats, administration of CI994 for 24 hours induced cells apoptosis in a dose-dependent manner (1 μM~10μM ) [1]. When tested with mitogen-stimulated blood lymphocytes, CI-994 treatment inhibited cells proliferation with IC50 value of 3 mM [5]. In NSCLC cell lines (A549 and LX-1), CI994 administration induced cell apoptosis via inhibiting HDAC activity and arresting cell cycle at G0/G1 phase and showed a promising therapy strategy when combined with chemotherapy [2]. In male rats model, oral administration of CI994 markedly effected lymphoid tissue via reducing blood lymphocytes, monocytes, neutrophils and bone marrow lymphoid cells at the concentration of 10, 23, and 45 mg/kg on day 1 and/or 3 [5].References: [1]. Graziano, M.J., et al., Induction of Apoptosis in Rat Peripheral Blood Lymphocytes by the Anticancer Drug CI-994 (Acetyldinaline)(*). J Biomed Biotechnol, 2001. 1(2): p. 52-61.[2]. Loprevite, M., et al., In vitro study of CI-994, a histone deacetylase inhibitor, in non-small cell lung cancer cell lines. Oncol Res, 2005. 15(1): p. 39-48.[3]. Cacan, E., et al., Inhibition of HDAC1 and DNMT1 modulate RGS10 expression and decrease ovarian cancer chemoresistance. PLoS One, 2014. 9(1): p. e87455.[4]. Beckers, T., et al., Distinct pharmacological properties of second generation HDAC inhibitors with the benzamide or hydroxamate head group. Int J Cancer, 2007. 121(5): p. 1138-48.[5]. Graziano, M.J., A.J. Galati, and K.M. Walsh, Immunotoxicity of the anticancer drug CI-994 in rats: effects on lymphoid tissue. Arch Toxicol, 1999. 73(3): p. 168-74.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
校准品：公司指定用校准某检测系统（仪器+试剂+程序）考虑具基质效应情况赋予校准品校准值校准品必须专用于某检测系统北京标准物质网：www.biaowu.com
我做实验的药物很难买到标准品，但现在有粗品，自己在提纯，想问下各位大牛，在没有标准品的情况下，怎么测提纯后该物质的纯度。谢谢
我们维克奇专门做标准品/对照品的,产品纯度要大于等于百分之九十八,可以看看我们的网站信息
例用微物测定孢克罗效价用孢克罗标准品用HPLC或UV测定则用照品；非西丁用作熔点校准物质用熔点标准品测定含量用照品即使同种物质标准品照品规格、标定及用途都能同（北京标准物质网）
