| Product Name | SensoLyte ® 520 Cathepsin D Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72170 |
| US$ | $504 |
Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease. The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL® 520/HiLyte Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations. The kit contains: HiLyte Fluor 488/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Assay buffer Bovine spleen Cathepsin D Inhibitor Fluorescence reference standard for calibration A detailed protocol Kit size: 100 assays (96-well plate) | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Storage | -20°C |
| Product Citations | Feng D. et al. (2011). High-Fat DietInduced Adipocyte Cell Death Occurs Through a Cyclophilin D Intrinsic Signaling Pathway Independent of Adipose Tissue Inflammation. Diabetes 60;2134Li, PP. et al. (2010). Functional Heterogeneity of CD11c-positive Adipose Tissue Macrophages in Diet-induced Obese Mice. J. Biol. Chem 285:15333.Buckow, R. et al. (2010). Bovine cathepsin D activity under high pressure. Food Chemistry 120;474. |
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校准品：公司指定用校准某检测系统（仪器+试剂+程序）考虑具基质效应情况赋予校准品校准值校准品必须专用于某检测系统北京标准物质网：www.biaowu.com
我做实验的药物很难买到标准品，但现在有粗品，自己在提纯，想问下各位大牛，在没有标准品的情况下，怎么测提纯后该物质的纯度。谢谢
我们维克奇专门做标准品/对照品的,产品纯度要大于等于百分之九十八,可以看看我们的网站信息
例用微物测定孢克罗效价用孢克罗标准品用HPLC或UV测定则用照品；非西丁用作熔点校准物质用熔点标准品测定含量用照品即使同种物质标准品照品规格、标定及用途都能同（北京标准物质网）
