Bovine Universal Reference cDNA-Oligo dT is a full length first strand cDNA synthesized from Zyagen universal reference total RNA which is isolated from wide variety of male and female major tissues including brain, heart, kidney, liver, lung, skeletal muscles, pancreas, skin, stomach, intestine, colon, testis, ovary and uterus.
The cDNA is primed with Oligo dT primer and can be used in gene cloning, target sequencing and as a control for gene expression analysis by regular PCR and real time PCR. Each cDNA product is enough for 40 PCR reactions.
Features:
- Synthesized from high quality intact universal reference total RNA
- Consistent control for standard data set comparisons
- Prepared from several major tissues for broad gene coverage
- Made at industrial scales to minimize lot-to-lot variation.
Quality Control: The integrity of each RNA sample used for cDNA synthesis is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also used as template PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.
Applications: cDNA is ideal as a control for gene expression analysis by real time and regular PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping: each universal reference cDNA sample is routinely shipped on dry ice in 1.5 ml vials and is enough for 40 reactions (40 PCR amplifications).
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
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Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM：标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度（BS,NISTBASARMI等）（ISO Guide 30-1992）
Reference Materials（RM）
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. （ISO Guide 30-1992）
照品RM：种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植（ISO Guide 30-1992）
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