Human Skeletal Muscles MicroRNA (<200nt) is enriched from total RNA extracted from freshly harvested tissues using classical guanidine isothiocyanate–phenol:chloroform extraction method and treated with DNase to remove residual DNA contamination. The small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA, precisely quantified, and stored at -80oC.
Quality Control: The integrity of each RNA sample is verified by denatured agarose gel electrophoresis to confirm that each preparation contains intact microRNA with virtually no large RNA contamination. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1).
Applications: MicroRNA is ideal for miRNA, siRNA, shRNA, and snRNA analysis by real time PCR, RT-PCR analysis, bioanalyzer, Northern Blot analysis, RNase protections assay, primer extension, and expression standard in microarray applications.
Packing/shipping: Total RNA sample is routinely provided in RNase-free water at a concentration of 0.25mg/ml or 0.5mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer. Samples are shipped on dry ice.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
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Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM：标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度（BS,NISTBASARMI等）（ISO Guide 30-1992）
Reference Materials（RM）
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. （ISO Guide 30-1992）
照品RM：种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植（ISO Guide 30-1992）
我们维克奇专门做标准品/对照品的,产品纯度要大于等于百分之九十八,可以看看我们的网站信息
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校准品：公司指定用校准某检测系统（仪器+试剂+程序）考虑具基质效应情况赋予校准品校准值校准品必须专用于某检测系统北京标准物质网：www.biaowu.com
各们前辈，初涉诊断行业，开发PCR诊断试剂，求问微生物标准品或者质控血清（比如支原体、衣原体、金葡菌等）应该到哪里购买啊？中检所和卫生部临检中心好像都没有。谢谢！
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