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Exocell/Glycated LDL/1009 Strip Plate
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4000-520-616
GlycatedLDL | |
Manufacturer/Trade: | Exocell/Glycacor |
CatalogueNumbers: | 1009StripPlate |
Methodology: | IndirectCompetitiveELISA |
Summaryofprocedure: | Non-rADIoactiveimmunoassayforthequantitativedeterminationofglycatedLDLinplasmatoevaluateglycemiccontrolindiabetes,andtoassessthecontributionofthemodifiedproteintovasculardisease.TheassaydependsuponES12,amonoclonalantibodydirectedagainstaspecificepitopeinglycatedLDL.TheglycatedLDLismeasuredinapolipoproteinBequivalents,andisexpressedasug/mlormg/dl.GlycacorisacompetitiveELISAinamicroplateformat. Tocompletetheassay,theES12isaddedtoamicrotiterplatethatcontainsimmobilizedglycatedLDLandsolubleglycatedLDL(standardorexperimentalsample).ThemonoclonalantibodybindstoeithertheimmobilizedglycatedLDLorthesolublephaseglycatedLDLduringtheprimaryincubation,hencethenotionofcompetitivebinding.Reactantsinthefluidphasearesubsequentlywashedaway,andcapturedES12onthesolidphaseisdetectedwithanenzymeconjugatedanti-mouseantibody.TheresultsaremadequantitativeafterchromogenicreactionwithTMB. |
SpecimenRequired: | 20ulCitrateorEDTAtreatedplasma |
AssayRange: | 3-40ug/ml(correspondingto0.3-4mg/dl) |
Precision: | IntraandinterassayprecisionofsampleswithintheusefulrangeoftheassayhaveaC.V.<10%. |
Specificity: | ES12isspecificforglycatedLDL,anddoesnotcross-reactwithotherhumanplasmaproteinsincludingnon-glycatedLDLineitherELISAorWesternBlotformats. |
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