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Exocell/The Creatinine Companion/1012 Strip Plate
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4000-520-616
TheCreatinineCompanion | |
Manufacturer/Trade: | Exocell/TheCreatinineCompanion |
CatalogueNumbers: | 1012StripPlate |
Methodology: | ChemicalAnalysis |
Summaryofprocedure: | TheCreatinineCompanionisachemicalassaydesignedtomonitorurinarycreatinineinaconvenientmicroplateformat.Itisintendedasa"companion"forExocellurinaryalbuminassays. TheassayisbasedupontheJaffe"reactionofalkalinepicratewithcreatinine.Standardsorsamplesareaddedtoamicroplate,andalkalinepicratereagentisadded.Absorbanceat500nmisdeterminedaftera10minuteincubationonthebenchtop.Subsequently,anacidsolutionisadded,andabsorbanceisagaindeterminedaftera5minuteincubation.Thedifferencebetweentheseabsorbancevaluesisdirectlyproportionaltothethecreatinineconcentration,andastandardcurveisgeneratedfromtheresponsetothestandards.Unknownsamplesareevaluatedbycomparingresponsetothestandardcurve.Theassaymaybecompletedinlessthan30minutes. Theassayservesasanormalizationprocedureforotheranalytesinurinesamples,particularlyforurinaryalbumin(1,2).Itallowsforameasureofkidneyfunctionbasedonaspoturinesampleratherthantheusual24hourpool. |
SpecimenRequired: | Urine,20ul |
AssayRange: | 10-100ug/mlor1-10mg/dL |
Precision: | Intra-andinterassayprecisionofsampleswithintheusefulrangehaveaC.V.<10%ofthemean. |
References: | 1.Bennet,P.H.et.al.Screeningandmanagementofmicroalbuminuriainpatientswithdiabetismellitus.Am.J.KidneyDis.25:107,1995. 2.Warram,J.H.et.al.Effectofdurationoftype1diabetiesontheprevalenceofstagesofdiabeticnephropathydefinedbyurinaryalbumin/creatinineratio.J.Am.Soc.Nephrol.7:930,1996. |
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