
As the simplified version of our most popular and powerful Avalanche®-Omni Transfection Reagent (Avalanche®-Omni), Avalanche®-Everyday Transfection Reagent (Avalanche®-Everyday) has the basic formulation of Avalanche®-Omni, thus is also an exceptionally powerful and versatile next-generation DNA and siRNA broad spectrum transfection reagent. Avalanche®-Everyday's transfection efficiency is very close to that of Avalanche®-Omni, whereas the price of Avalanche®-Everyday is only 1/3 that of Lipofectamine® 2000 (L2K). In addition, Avalanche®-Everyday is able to do 3000 x 24-well transfections/1.5 ml as compared to 750-1000 x 24-well transfections/1.5 ml for L2K.
Features:
- Broad Spectrum DNA/siRNA delivery- one transfection reagent and protocol for a variety of cells.
- Transfection efficiency is close to that of Avalanche®-Omni Transfection Reagent, but with much lower prices. Its price is only 1/3 that of L2K, and 1/3.4 that of Lipofectamine® 3000 (L3K).
- In addition, It is able to do 3000 x 24-well transfections/1.5 ml as compared to 750-1000 x 24-well transfections/1.5 ml for L2K
- Very low Cellular Toxicity because of its bio-degradability after endocytosis. Maintain cell density, reduce experimental biases.
- Same simple protocol as that of Omni: Does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.
- High levels of recombinant protein production
- Ideal for high-throughput work
- A must-have transfection reagent for everyday use on commonly used cells
EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商,公司的研发宗旨是让生命科学研究变得更简单,快速,容易!该公司主要产品之一为新一代转染试剂Avalanche 系列产品,该产品集合了组合化学,分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成,包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。
EZBiosystems特约代理EZBiosystems品牌名称:EZBiosystemsEZBiosystems品牌logo:EZBiosystems品牌官网:http://ezbiosystems.com/EZBiosystems品牌链接:http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍:EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品:品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml
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一、免疫组化试剂盒一般包括:
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别,所以购买时一定了解清楚:
1、一抗是做人的还是兔、鼠的组织;
2、检测系统是ABC/SP/非生物素/其他;
3、试剂盒的内容都有什么?有没有显色系统?
4、如果没有,自己根据前面的检测系统需配哪个显色系统。(其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配)
三、免疫组化抗体即指特异性一抗,是用来标记的指标,免疫组化过程的其他试剂一律另外配备。
另外:做实验时,如遇出结果困难,记得做阳性片对照,敢于怀疑检测系统或显色系统的问题。
用双氧水去除过氧化物酶时,我是自己配的3%双氧水滴到片上而非将片浸入双氧水,这样可否?
用博士德的DAB染色,配制方法为:1mlH20中加入DAB、H2O2、TBS各一滴,我配制的时候发现将DAB加入H20时很容易沉淀,要震荡才能混匀,且将配好的DAB滴到玻片上时,DAB会结为很小块的微颗粒悬浮在液体中但并不染色,是否DAB有问题?
现在打算换中衫的试剂盒,代理商说中衫的试剂盒包括了二抗和DAB,想请教一下该试剂盒的具体内容有些什么?
万分感谢!
上海好多的供应商的,具体还是看你选择了,其实试剂盒产品都差不多,什么远慕生物,古朵生物...好多的额

