PRODUCT SPECIFICATIONS
ITEM# 4003: Recombinant HCV 20kD Core Protein (Pichia)
SPECIFICATIONS APPLICATIONS
Concentration: 500ug/ml In-Vitro HCV Diagnostics
Mass/vial: 100ug T-Cell Activation
Volume/vial: 200ul
Diluent: 20mM Citrate-Phosphate, pH6.0, 0.5M Urea
Purity : >90%
Stabilizer: None
Preservative: None
Storage: -75o C
Stability: At least 6 months at -75o C
DESCRIPTION: Full length HCV p22 capsid his-tag protein expressed in the Pichia pastori Expression System.
PURIFICATION: HCV core fusion protein is purified by Ni-affinity chromatography methods to > 90% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HCV converted human serum polyclonal antibodies in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: Not determined.
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 50 to 100-fold in suitable buffer for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
HCV core protein shows relatively strong biding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.
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一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
用双氧水去除过氧化物酶时，我是自己配的3%双氧水滴到片上而非将片浸入双氧水，这样可否？
用博士德的DAB染色，配制方法为：1mlH20中加入DAB、H2O2、TBS各一滴，我配制的时候发现将DAB加入H20时很容易沉淀，要震荡才能混匀，且将配好的DAB滴到玻片上时，DAB会结为很小块的微颗粒悬浮在液体中但并不染色，是否DAB有问题？
现在打算换中衫的试剂盒，代理商说中衫的试剂盒包括了二抗和DAB，想请教一下该试剂盒的具体内容有些什么？
万分感谢！
上海好多的供应商的，具体还是看你选择了，其实试剂盒产品都差不多，什么远慕生物，古朵生物...好多的额
