PRODUCT SPECIFICATIONSProduct # 15015: Recombinant Treponema pallidum 15kD Antigen(E.coli)SPECIFICATIONS APPLICATIONSConcentration: 1mg/ml ElisaMass/vial: 1mg Western ElisaDiluent: PBS, pH7.2. DiagnosticsPurity : >95%Stabilizer: NonePreservative: NoneStorage: -75 o CStability: At least 12 months at -75 o C
DESCRIPTION: T. pallidium full length recombinant 15kD antigen produced in E.coli expression system.
PURIFICATION: T. pallidium 15kD antigen is purified by immune-affinity and ion-affinity chromatography methods to >95% purity as determined by SDS-PAGE, reduce.
SPECIFICITY: This protein binds murine monoclonal antibodies and T. pallidium converted humanpolyclonal antibodies in ELISA and Western ELISA. Used for diagnosis of syphilis.BIOLOGICAL ACTIVITY: Not determined.APPLICATION AND INSTRUCTIONS FOR USERecommended concentrations for use are approximate values. Dilute the antigen preparation at least 1-5ug/ml in PBSfor solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determinethe optimal concentration for use in specific applications.T.pallidium 15kD antigen antigen shows relatively strong binding specificity for specific human antibodies.ELISA and Western ELISA require protein in 50ng range depending on the nature and affinity of thesecondary detection reagent. T. pallidium converted human polyclonal antibodies yield titers of 1:2 000 orgreater at 100ng of immobilized protein under standard
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点：我看了Elisa的说明书，现在检测PDGF有两种试剂盒，一种是检测PDGF的，要求的试剂样本为组织匀浆；一种是检测PDGF-BB的，要求的待测样本为血液或血浆。
我现在的实验目的是想更精确，检验组织中的PDGF-BB，但是手中的是人体韧带组织样本，非PDGF-BB试剂盒所要求的组织样本为血浆或血清，我不知道是否可以用PDGF-BB的ELISA试剂盒的来做，所以特地请教：）
请教第二点：市面上有RB公司的96T，2500元；另外有Uscnlife的Elisa试剂盒，96T的3680元，都说是原装进口的，我该如何选择呢？为何差价这么多？谢谢指点！
