The EpiScope MSP Kitfeatures a reagent designed exclusively for DNA methylation analysis via methylation-specific PCR (MSP) (Herman et al. 1996). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyseson bisulfite-treated, uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring using TB Green, enabling the performance of both real-time and endpoint PCR under the same reaction conditions.
The EpiScope MSP Kitfeatures a reagent designed exclusively for DNA methylation analysis via methylation-specific PCR (MSP) (Herman et al. 1996). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyseson bisulfite-treated, uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring using TB Green, enabling the performance of both real-time and endpoint PCR under the same reaction conditions.
Principle of MSP
Methylation-specific PCR identifies nucleotide regions in which cytosines have been altered to uracils following bisulfite treatment of the DNA template. These alterations are a function of the DNA template"s CpG methylation status; methylated cytosines remain unaltered while the unmethylated cytosines are altered to uracils. Two primers are designed to detect these unaltered or altered regions, the methylated CpG DNA primer (M primer) and the unmethylated CpG DNA primer (UM primer), respectively. The last step involves methylation-specific PCR.
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一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
用双氧水去除过氧化物酶时，我是自己配的3%双氧水滴到片上而非将片浸入双氧水，这样可否？
用博士德的DAB染色，配制方法为：1mlH20中加入DAB、H2O2、TBS各一滴，我配制的时候发现将DAB加入H20时很容易沉淀，要震荡才能混匀，且将配好的DAB滴到玻片上时，DAB会结为很小块的微颗粒悬浮在液体中但并不染色，是否DAB有问题？
现在打算换中衫的试剂盒，代理商说中衫的试剂盒包括了二抗和DAB，想请教一下该试剂盒的具体内容有些什么？
万分感谢！
