Overview
The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
- Rapid – Ready for PCR in 15 minutes
- Convenient – No DNA extractions
- Reliable – Consistent amplification results
Specifications
For Research Use Only. Not for use in diagnostic procedures.
Protocol and Resources
Product Documentation & Literature
PROTOCOL
TQ2310 Tissue Direct PCR Kit
Product Data
E.Z.N.A.® Tissue Direct PCR Kit worked well for various tissue samples.
Figure 1. Dealing with the pig, chicken, and fish samples with the E.Z.N.A.® Tissue Direct PCR Kit and amplification using the DNA as template respectively, 12% PCR products were loaded onto a 1% agarose gel. M: DL2000 DNA marker, C1: positive control, C2: negative control, using ddH20 as template.
E.Z.N.A.® Tissue Direct PCR Kit performance with different human sample types
Figure 2. Dealing with the human sample with the E.Z.N.A.® Tissue Direct PCR Kit and amplification using the DNA as template respectively, 10% PCR products were loaded onto a 1% agarose gel. M: DL2000 DNA marker, C1: positive control, C2: negative control, using ddH20 as template.
