
Generally, low molecular weight peptides and synthetic peptides do notspontaneously attach to 96-well microtiter ELISA plates. The Peptide Coating Kit is designed to allow optimal peptide plate coatingonto96-well plates. The Peptide Coating Kit also includes reaction buffer, 96-well microplates, and blocking solution designed specifically for subsequent ELISA.
Principle
The free carboxyl groups of peptides used for plate coating can be covalently linkedvia reaction with the water-soluble peptide coupling reagent l-ethyl-3-(3-(dimethylamino)-propy1)carbodiimide (EDC). These peptides can subsequently belinkedto amino groups thatare attachedto the surface of microtiter plate wells. The end result is a population ofpeptides directly coating the surface of the microtiter platevia peptide bonds.
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一、免疫组化试剂盒一般包括:
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别,所以购买时一定了解清楚:
1、一抗是做人的还是兔、鼠的组织;
2、检测系统是ABC/SP/非生物素/其他;
3、试剂盒的内容都有什么?有没有显色系统?
4、如果没有,自己根据前面的检测系统需配哪个显色系统。(其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配)
三、免疫组化抗体即指特异性一抗,是用来标记的指标,免疫组化过程的其他试剂一律另外配备。
另外:做实验时,如遇出结果困难,记得做阳性片对照,敢于怀疑检测系统或显色系统的问题。

