The EpiScope Nucleosome PreparationKit is designed for the preparation of nucleosomes from cultured mammalian cells. DNAis extracted from the prepared nucleosomes and analyzed using real-time PCR or sequencing to map nucleosome positions.
The EpiScope Nucleosome PreparationKit is designed for the preparation of nucleosomes from cultured mammalian cells. DNAis extracted from the prepared nucleosomes and analyzed using real-time PCR or sequencing to map nucleosome positions.
Nucleosomes are the constituent units of chromatin in eukaryotic nuclei, consisting of genomic DNA wrapped around a core histone complex (Clark 2010). Nucleosomes are not spaced at regular intervals on genomic DNA; instead,their positions aredependent on chromatin structure and transcriptional regulation (Dutta and Workman 2012). In DNA methylation analysis, an important area inepigenetics research, nucleosome positioning has become a subject of intense interest. In transcriptionally active genes, the promoter region is free of nucleosomes,permitting the binding of transcriptional initiation factors (TIFs). Conversely, DNA methylationwithingene promoter regions leads to the formation of nucleosomes in those regions, preventing the binding of TIFs andsuppressing transcriptional initiation.
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一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
