- Description
- Additional
Description
Details
Description:Mouse Immunoglobulin G2b (IgG2b) is prepared from normal mouse serum.
Purification:Affinity column
Formulation:Lyophilized from a solution of 0.01M PBS, pH7.4
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.
Storage: Store at -20oC
Applications:Research and further manufacturing
References: If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | Mouse Immunoglobulin G2b (IgG2b) |
|---|---|
| Application | Research & Further Manufacturing |
| Size | 10 mg |
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有没有朋友使用碧云天的细胞衰老β-半乳糖苷酶染色试剂盒做过细胞衰老,本人小白,求相关细胞处理的过程及数据分析方法。贴壁细胞六孔板需要接种多少,染色后怎样计数进行数据处理。谢谢啦!
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注,图注的意思我明白,但是我想知道merge后的图看什么颜色的荧光,蓝色是细胞核,红色是lysosome(位于胞质),绿色是AMPKβ2,该实验是想观察AMPKβ2是否转位到lysosome上了,如果确实发生了AMPKβ2转位到lysosome上,那么merge后是红色与绿色融合在一起,是吗?融合在一起发什么颜色的光了?
本人对软骨石蜡切片做了MMP-13的免疫组化染色,首次做,无法判断染色部位,请各位帮忙看一下
(2)另一方面,使抗原抗体结合更稳定。一般不需要,但对表达较弱的抗原可能有用,4度和37度时分子运动方式不同,前者分子碰撞机率和运动速度小于后者,后者结合更快,但敏感性也提高了并易造成非特异染色。
(3)其实,我更赞同后一种说法,因为我尝试把肝脏或睾丸片子从4度过夜拿出后,直接用PBS洗没发生过脱片现象。事实胜于雄辩!

