Type:Protein
Applications:WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid-liquid
Immunogen:RecombinantartificialmosaicproteincontainstheCMVgBimmunodominantregions,60-81aaofgBproteinstrainAD169repeatedthreetimes.
CMVbelongstotheBetaherpesvirinaesubfamilyofHerpesviridaewhichincludesherpessimplexvirustypes1and2,varicella-zostervirus,andEpstein-Barrvirus.TheherpesvirusesshareacharacteristicABIlitytoremainlatentoverlongperiods.CMVisadouble-strandedlinearDNAviruswith162hexagonalproteincapsomeressurroundedbyalipidmembrane.CMVhasthelargestgenomeoftheherpesviruses,rangingfrom230-240kilobasepairs.
HumanCMViscomposedofuniqueandinvertedrepeatsthatincludetheexistenceof4genomeisomerscausedbyinversionofL-Sgenomecomponents(classE).Replicationmaybedividedintoimmediateearly,delayedearly,andlategeneexpressionbasedontimeofsynthesisafterinfection.TheDNAisreplicatedbyrollingcircles.Invitro,CMVreplicatesinhumanfibroblasts.
Specificity:ImmunoreactivewithseraofCMV-infectedindividuals.
Applications:CMVMosaicantigenissuitableforELISAandWesternblots,excellentantigenfordetectionofCMVwithminimalspecificityproblems.
OtherReagentstoConsider:
CytomegaloVirusPp28(UL99)
ebiomall.com
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可与MHCⅡ类分子结合的都是蛋白性抗原;多糖和脂类不易于MHCⅡ类分子连接,难以被TH细胞识别,因而多不是良好的免疫原;但有时可以诱导抗体性免疫应答。 抗原递呈(antigenpresentation)是辅佐细胞向辅助性T细胞展示抗原和MHCⅡ类分子的复合物,并使之与TCR结合的过程。这个过程是几乎所有淋巴细胞活化的必需步骤。抗原递呈之前,经处理后的抗原肽段已经连接在MHC分子顶端的槽中,这个复合物便是TCR的配体。TCR与配体结合的精确模式尚未清楚,一个合理的说法是TCR中α和β链的V段接触MHC分子的α螺旋(形成MHC分子顶端槽的肽段),使高可变的连接部(V-J及V-D-J)与抗原肽段相结合。这样保证了TCR识别抗原的特异性。
超抗原的递呈有独特的模式,它不需要胞内处理,可以直接与MHCⅡ类分子结合。超抗原不结合在MHCⅡ类分子的顶端槽中,而是结合在槽的外侧;与TCR结合时,不结合其α链,只结合β链的V节段。超抗原对TCR和MHCⅡ类分子的结合都非常牢固,象一支双向钩子将T细胞和辅佐细胞紧紧地连在一起,很容易使T细胞活化。另外,任何超抗原都只与含特殊β链V节段的TCR结合,这样的TCR约占外周T细胞总数的1%~10%,这一数字远远大于任何普通抗原所能识别的细胞数;所以某些产毒细胞感染时,容易发生急性期素休克综合征,就是超抗原刺激的结果。向左转|向右转
抗体(antibody)
看了百科以后依然不太明白,语言通俗一点最好。谢谢。