·DesigningAntigens(Perkin-Elmer)WhatisanEpitope?ChoosingtheEpitopeMethodsforEpitopePredictionDesigningtheSyntheticPeptidePromiscuousT-cellEpitopes ·AntigenDesign&SeraPurification(Genosys)Thisprotocoldescribespeptideselectionanddesign,couplingstrategy,andcarrierproteinswhichareimportantfactorsinanti-peptideantiserageneration. ·PreparationOfPeptide-KLHConjugatesForImmunization(MitchisonLab) ·TestingRabbitBleedsByELISA(MitchisonLab)YoucantestwhetherornotyouhavegottenanimmuneresponsetothepeptideandhowstrongthatimmuneresponseisbydoingELISAsagainstpeptideconjugatedtoBSA.