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GenWay/Mouse IgG Antibody (Biotin)/GWB-429DDB/1 mg
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4000-520-616
AdditionalInformation:
| Name | MouseIgGAntibody(Biotin) |
|---|---|
| RelatedProductNames | RABBITF(ab")2ANTIMOUSEIgG:BiotinIgGIGHG1 |
| GeneSymbol | Ighg1 |
| GeneFamily | IGH |
| Geneid | 16017 |
| AliasSymbols | IgG1,Igh-4,VH7183,Ighg1 |
| ProteinName | Iggamma-1chainCregion,membrane-boundform |
| DescriptionofTarget | RABBITF(ab")2ANTIMOUSEIgG:Biotin |
| SwissprotID | P01869 |
| SpeciesReactivity | Mouse |
| Host | Rabbit |
| Isotype | PolyclonalIgG |
| Conjugation | Biotin |
| Replacement | Thisantibodymayreplaceitemsc-52003fromSantaCruzBiotechnology. |
| Immunogen | PurifiedmouseIgG |
| Homology | Mouse |
| ProductFormat | F(ab")2fragmentofIgGconjugatedtobiotin-liquid |
| SwissProtNumber | P01869 |
| Specificity | IgG |
| Concentration | 1.0mg/ml |
| Applications | C,E,F,P |
| ApplicationInfo | FlowCytometry: 1/50-1/100 Immunohistology-Frozen: 1/50-1/200 Immunohistology-Paraffin: 1/50-1/200 ELISA: 1/1000-1/2000 |
| Clonality | Polyclonal |
| Format | Solution:Phosphatebufferedsaline,Stabalizer:0.09%,SodiumAzide,Form:F(ab")2fragmentofIgGconjugatedtobiotin-liquid |
| StABIlity | 18monthsfromdateofdespatch. |
| ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrost-freefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
| Reactivity | Mouse |
| Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrost-freefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitate |
| ProtocolInformation | Citation:1:TurnerOC,BasarabaRJ,OrmeIM.ImmunopathogenesisofpulmonarygranulomasintheguineapigafterinfectionwithMycobacteriumtuberculosis.InfectImmun.2003Feb;71(2):864-71.PubMedPMID:12540568;PubMedCentralPMCID:PMC145351. Species:Guineapigs,M.tuberculosis ExperimentName:Immunohistochemistry ExperimentBackground:1.Localizationoflymphocytesubsetsbyimmunohistochemistry.2.Pulmonarytuberculosisinguineapigsissimilartothediseaseinhumansandisaccordinglywidelyusedasamodeltotesttuberculosisvaccines.Theprimarysiteofexpressionofacquiredimmunityandthehallmarkoftuberculosisisthegranuloma.Granulomamorphologyiswelldescribed,butthereislimitedinformationregardingT-cellsubsetinflux.Oliverandhisteammonitoredthecourseofpulmonarytuberculosisinguineapigsandobservedfourdistinctimmunohistopathologicalstages.InallstagesthereweresimilarnumbersandarrangementofCD4andCD8Tcells.Therewereonlysmallnumbersofapoptoticlymphocytes,scatteredaroundandwithinthenecroticcore,andacid-fastbacilliwerevisIBLebothwithinmacrophagesandfreewithinairwaydebris.Akeyfindingofthestudywastheobservationthatthedevelopmentofthenecroticcorewasanearlyeventandalmostcertainlyprecededtheemergenceoftheacquiredimmuneresponse.Thisinturnsuggeststhatinnatemechanismsarethebasisoftheearlylesionsandthatsubsequentacquiredresponsesareunabletomoderatethem.ThishypothesisdiffersfromthecurrentdogmathatexcessiveactivityofTcellsmediatesdelayed-typehypersensitivityandthatcellularcytolysisistherootcauseofthenecrosis. ExperimentalSteps:Mouseanti-guineapigmonoclonalantibodiesspecificforguineapigCD4(cloneCT7)andCD8(cloneCT6)werepurchasedfrom(Oxford,England).Ineachcase,themouseimmunoglobulinG1(IgG1)-negativecontrol(cloneW3/25)wasusedastheisotypecontrol.F(ab’)2rabbitanti-mouseIg-biotin,alsofromwasusedasthesecondaryantibodyAtnecropsy,theleftcraniallunglobewasfirstslowlyinfusedwitha20%OCT(Tissue-Tek,Inc.Torrance,Calif.)–phosphate-bufferedsalinesolutionthroughthemajorvesselsatthehilus.Thesampleswerethenplacedinatissuemold,completelysurroundedbyOCT,frozeninabathofliquidnitrogen,andstoredat-70°Cuntilused.Serialsectionsfromeachlung,5µmthick,werecutinacryotome(CM1850;Leica,Bannockburn,Ill.)byemployingtheInstrumedicsInc.(Hackensack,N.J.)tapetransfersystem,fixedincoldacetonefor5to10min,andthenairdried.Next,thesectionswerewashedinAPKbuffersolution(VetanaMedicalSystems,Tucson,Ariz.)for15to20minandincubatedina1:50(CD4)or1:100(CD8)solutionofProteinBlockgoatserum(Biogenex,SanRamon,Calif.)andprimaryantibodyfor30min.ThesectionswerethenplacedonaNexusautomatedimmunostainer(VentanaMedicalSystems).Thelabeledavidin-alkalinephosphataseandFastred/naptholdetectionkitwasemployed.Thesecondaryantibodywasincubatedfor30minatroomtemperature.SectionswerecounterstainedwithMeyer’shematoxylin.Sectionsofspleenwerealsoexaminedtoactaspositivecontrols. NumberOfProtocols:1 |
| LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
| IntendedUse | ResearchUseOnly |
| KeyReference | 1.Silveira,M.etal.(2002)InfectionwithStrongyloidesVenezuelensisinducestransientairwayeosinophilicinflammation,anincreaseinimmunoglobulinEandhyperresponsivenessinrats.Infect.Immun.70(11):6263-6272 |
| :: | PreservativeStabilisers:0.09%-SodiumAzide AntiserumPreparation:AntiseratoMouseIgGwereraisedbyrepeatedimmunisationofrabbitswithhighlypurifiedantigen.PurifiedIgGwaspreparedfromwholeserumbyaffinitychromatography.F(ab")2fragmentswerepreparedbypepsindigestionoftheIgGfollowedbyagelfiltrationsteptoremovetheremainingintactIgGorFcfragments. |
| :: | ApproxProteinConc:F(ab")2concentration1.0mg/ml BufferSolutions:PhosphatebufferedsalinepH7.2 |
