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GenWay/Mouse IgG1 Antibody (HRP)/GWB-5419A3/1 ml
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4000-520-616
Description:
ShortDescription:GOATANTIMOUSEIgG1HRPAdditionalInformation:
| Name | MouseIgG1Antibody(HRP) |
|---|---|
| RelatedProductNames | GOATANTIMOUSEIgG1:HRP |
| GeneSymbol | Ighg1 |
| GeneFamily | IGH |
| Geneid | 16017 |
| AliasSymbols | IgG1,Igh-4,VH7183,Ighg1 |
| ProteinName | Iggamma-1chainCregion,membrane-boundform |
| DescriptionofTarget | GOATANTIMOUSEIgG1:HRP |
| SwissprotID | P01869 |
| SpeciesReactivity | Mouse |
| Host | Goat |
| Isotype | PolyclonalIgG |
| Conjugation | HRP |
| Replacement | Thisantibodymayreplaceitemsc-52003fromSantaCruzBiotechnology. |
| Immunogen | MouseIgG1paraproteins |
| Homology | Mouse |
| ProductFormat | PurifiedIgGconjugatedtoHorserADIshPeroxidase(HRP)-liquid |
| SwissProtNumber | P01869 |
| Specificity | IgG1 |
| Applications | C,E,P,WB |
| ApplicationInfo | ELISA: 1/4000-1/8000 |
| Clonality | Polyclonal |
| Format | Solution:Phosphatebufferedsaline,Stabalizer:0.01%Thiomersal,50%Glycerol,Form:PurifiedIgGconjugatedtoHorseradishPeroxidase(HRP)-liquid |
| StABIlity | 18monthsfromdateofdespatch. |
| ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrost-freefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
| Reactivity | Mouse |
| Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrost-freefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitat |
| ProtocolInformation | Citation:1:AndersenP.EffectivevaccinationofmiceagainstMycobacteriumtuberculosisinfectionwithasolublemixtureofsecretedmycobacterialproteins.InfectImmun.1994Jun;62(6):2536-44.PubMedPMID:7910595;PubMedCentralPMCID:PMC186542. Species:Mouse,Bacteria ExperimentName:AntibodytiterstoST-CFofdifferentIgGisotypes. ExperimentBackground:TherelativelevelsofspecificIgGlandIgG2AinducedbythedifferentdosesofST-CFwereusedtogiveanindirectindicationofthelymphocytesubsetsinvolved. ExperimentalSteps:1.Serumsamplesobtainedfromthemice5weeksafterthefirstimmunizationwereanalyzedintwofolddilutionsonELISAplatescoatedwithST-CF(100µlperwell;5,ug/ml).2.Theplateswereincubatedfor2hatroomtemperature,and,afterwashing,theplatesreceivedperoxidase-labeledgoatantimouseimmunoglobulinG(IgG),IgG2A(1/1,000),orIgGl(1/2,000)(Kidlington,Oxford,UnitedKingdom).3.AntibodytiterstoST-CFofdifferentisotypesareexpressedasthedilutionsthatgaveanabsorbencyof1(IgGl)or0.4(IgG2A).4.ThetitershavebeenadjustedfordifferentlevelsofspecificIgGinducedbythevaccinesandarethereforepresentedasrelativeisotypetiters. OtherReagentsUsed:DDA,phosphatidylcholindistearoyl,TDM NumberOfProtocols:1 |
| LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
| IntendedUse | ResearchUseOnly |
| :: | PreservativeStabilisers:0.01%Thiomersal 50%Glycerol AntiserumPreparation:AntiseratomurineIgG1wereraisedbyrepeatedimmunisationofgoatswithpurifiedantigen.PurifiedIgGwaspreparedbyaffinitychromatography. |
| :: | BufferSolutions:PhosphatebufferedsalinepH7.4 |
