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GenWay/Mouse IgG1 Antibody (FITC)/GWB-5F3E80/0.5 mg
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4000-520-616
Description:
ShortDescription:GOATANTIMOUSEIgG1FITCAdditionalInformation:
| Name | MouseIgG1Antibody(FITC) |
|---|---|
| RelatedProductNames | GOATANTIMOUSEIgG1:FITC |
| GeneSymbol | Ighg1 |
| GeneFamily | IGH |
| Geneid | 16017 |
| AliasSymbols | IgG1,Igh-4,VH7183,Ighg1 |
| ProteinName | Iggamma-1chainCregion,membrane-boundform |
| DescriptionofTarget | GOATANTIMOUSEIgG1:FITC |
| SwissprotID | P01869 |
| SpeciesReactivity | Mouse |
| Host | Goat |
| Isotype | PolyclonalIgG |
| Conjugation | FITC |
| Replacement | Thisantibodymayreplaceitemsc-52003fromSantaCruzBiotechnology. |
| Immunogen | MouseIgG1paraproteins |
| Homology | Mouse |
| ProductFormat | PurifiedIgGconjugatedtoFluoresceinIsothiocyanateIsomer1(FITC)-liquid |
| SwissProtNumber | P01869 |
| Specificity | IgG1 |
| Concentration | 1.0mg/ml |
| Applications | C,F,IF |
| ApplicationInfo | FlowCytometry: Neat-1/10 Immunofluorescence: Neat-1/10 |
| Clonality | Polyclonal |
| Format | Solution:Phosphatebufferedsaline,Stabalizer:0.1%SodiumAzide,Form:PurifiedIgGconjugatedtoFluoresceinIsothiocyanateIsomer1(FITC)-liquid |
| StABIlity | 18monthsfromdateofdespatch. |
| ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrostfreefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
| Reactivity | Mouse |
| Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrostfreefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight.Avoidrepeatedfreezingandthawingasthism |
| ProtocolInformation | Citation:1:LaiWK,SunPJ,ZhangJ,JenningsA,LalorPF,HubscherS,McKeatingJA,AdamsDH.ExpressionofDC-SIGNandDC-SIGNRonhumansinusoidalendothelium:aroleforcapturinghepatitisCvirusparticles.AmJPathol.2006Jul;169(1):200-8.PubMedPMID:16816373;PubMedCentralPMCID:PMC1698775. Species:Human ExperimentName:1.ImmunohistochemistryandDual-ColorCo-Immunofluorescence2.SolubleHCVE2BindingandBlockingAssay ExperimentBackground:1.ImmunofluorescentstainingofmannosereceptorandCD68(green)wasusedtodetectKupffercells,andDC-SIGNstainedred.ColocalizationofthetworeceptorsisseenasyellowstainingconfirmingthatKupffercellsexpressDC-SIGNintheliver2.ToassesswhetherDC-SIGNandDC-SIGNRexpressedinnormalliverareabletointeractwithHCVE2,intactliversectionswereincubatedwithtruncatedE2fromHC-J4E2661andboundantigenvisualizedwithratanti-E2antibody9/75,whichrecognizestheCD81-bindingsiteonE2andhencefailstointeractwithE2-CD81complexes. ExperimentalSteps:1.ImmunohistochemistryandDual-ColorCo-Immunofluorescence:Thefollowingprimaryantibodieswereused:DC-SIGN(MAB161,IgG2b)andDC-SIGNR(MAB162,IgG2b)fromR&DSystems,LYVE-1(8C,IgG1;agiftfromDavidJackson,UniversityofOxford),CD68(EBM11,IgG1;fromAbcam,Cambridge,UK),andmannosereceptor(MCA2155,IgG1;fromLtd.,Oxford,UK).5-umcryostatsectionsderivedfromnormalliverwerefixedinacetonefor10minutesandstainedusingastandardalkalinephosphataseanti-alkalinephosphatasetechnique.Briefly,primaryantibodywasfollowedbyrabbitanti-mousemonoclonalandmousemonoclonalalkalinephosphataseanti-alkalinephosphatase(Dako).ThestainwasdevelopedwithfastredandnaphtholAS-MXphosphatesubstrate(Sigma-Aldrich).Sectionsfordualimmunofluorescencewereprewettedwithstainingbuffer(phosphate-bufferedsalinecontaining10%fetalcalfserumand0.1%sodiumazide)for10minutes.Slideswereincubatedwithprimaryantibodiesdilutedinstainingbufferfor1hourinahumidifiedchamber.Controlsectionswereincubatedwithisotype-matchedIgG2borIgG1(R&DSystems).Sectionswerestainedwithgoatanti-mouseIgG2bAlexaFluor(Molecularprobes,Eugene,OR)andgoatanti-mouseIgG1fluoresceinisothiocyanate.ImmunofluorescencewasassessedusingAxioVisionsoftware(CarlZeissMicroImaging,Inc.,Jena,Germany).2.SolubleHCVE2BindingandBlockingAssay:293-TcellsweretransientlytransfectedwithplasmidsexpressingHC-J4E2661orvectoralone(controlmockantigen)withLipofectamine2000(Invitrogen)accordingtothemanufacturer’sinstructions.TissueculturesupernatantscontainingHC-J4E2661wereharvested48hoursaftertransfection,andtheamountofHC-J4E2antigenwasquantifiedbyenzymeimmunoassay.NormalliversectionswerepreincubatedwithisotypematchedIgG2b(controlblock),DC-SIGN,DC-SIGNR(5ug/ml;bothfromR&DSystems),ormannan(20ug/ml;Sigma-Aldrich)for1hour,washedandthenincubatedwithmockantigenorHC-J4E2661atasaturatingconcentrationinphosphate-bufferedsaline/1%fetalbovineserum/0.05%sodiumazide/1mmol/LCaCl2for1houratroomtemperature,washed,andlabeledwithratanti-E2mAb9/75,whichrecognizestheCD81-bindingsiteonE2andhencefailstointeractwithE2-CD81complexes.Detectionofbindingonnormalliversectionwasasdescribedaboveusingimmunofluorescencewithgoatanti-ratfluoresceinisothiocyanate.ImmunofluorescencewasassessedusingAxioVisionsoftware(CarlZeissMicroImaging,Inc.). NumberOfProtocols:2 |
| LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
| IntendedUse | ResearchUseOnly |
| :: | PreservativeStabilisers:0.09%SodiumAzide(NaN3) AntiserumPreparation:AntiseratomouseIgG1wereraisedbyrepeatedimmunisationofgoatswithpurifiedantigen.PurifiedIgGwaspreparedfromwholeserumbyaffinitychromatography. |
| :: | ApproxProteinConc:IgGconcentration1.0mg/ml BufferSolutions:PhosphatebufferedsalinepH7.4 |
