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GenWay/Rat IgG2a Antibody (FITC)/GWB-6094FA/0.5 mg
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4000-520-616
Description:
ShortDescription:GOATANTIRATIgG2aFITCAdditionalInformation:
| Name | RatIgG2aAntibody(FITC) |
|---|---|
| RelatedProductNames | GOATANTIRATIgG2a:FITCIgG2aIgg-2a |
| GeneSymbol | LOC679045 |
| Geneid | 314494 |
| ProteinName | Iggamma-2AchainCregion |
| DescriptionofTarget | GOATANTIRATIgG2a:FITC |
| SwissprotID | P20760 |
| SpeciesReactivity | Rat |
| Host | Goat |
| Isotype | PolyclonalIgG |
| Conjugation | FITC |
| Homology | Rat |
| ProductFormat | PurifiedIgGconjugatedtoFluoresceinIsothiocyanateIsomer1(FITC)-liquid |
| SwissProtNumber | P20760 |
| Specificity | IgG2a |
| Concentration | 1mg/ml |
| Applications | F |
| ApplicationInfo | FlowCytometry: 1/500 |
| Clonality | Polyclonal |
| Format | Solution:Phosphatebufferedsaline,Stabalizer:0.09%,SodiumAzide.0.2%,BovineSerumAlbumin,Form:PurifiedIgGconjugatedtoFluoresceinIsothiocyanateIsomer1(FITC)-liquid |
| StABIlity | 18monthsfromdateofdespatch. |
| ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrost-freefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
| Reactivity | Rat |
| Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrost-freefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight.Avoidrepeatedfreezingandthawingasthism |
| ProtocolInformation | Citation:1:LópezMC,HolmesN.PhenotypicalandfunctionalalterationsinthemucosalimmunesystemofCD45exon9KOmice.IntImmunol.2005Jan;17(1):15-25.Epub2004Nov22.PubMedPMID:15557316. Species:Mouse ExperimentName:1.Flowcytometricstaining2.ImmunomicroscopyofPeyer’spatchandmesentericlymphnodecells ExperimentBackground:Exon9CD45KOmicepossessacompleteCD45nullphenotypeinthymus,spleenandlymphnodes.ItwasestablishedbyusingbothflowcytometryandtissuestainingthatCD45isabsentfromintestinalintraepitheliumandlaminaproprialymphocytes.CellsrecoveredfromCD45KOPP(Peyer’spatch)andMLN(mesentericlymphnode)werelargerthancellsfromnormalmice ExperimentalSteps:1.Flowcytometricstaininga)SmallintestineIELsfromCD45KOandnormalmicewerestainedwithratanti-mouseCD45(cloneYBM42.2)followedbyaFITC-conjugatedmouseanti-ratIgG2ashowingacompletelynullphenotype.b)SmalliIELswereisolatedandstainedwithdirectlyconjugatedantibodiesbeforebeinganalyzedinaFACScan.2.ImmunomicroscopyofPeyer’spatchandmesentericlymphnodecellsCytospinswerepreparedusingPPandMLNcellsinaShandoncytocentrifuge(ThermoShandon,Pittsburgh,PA).Theywereair-dried,fixedinmethanol,air-driedandstoredat-80°Cuntilstained.AfterstainingCytospinswereincubatedforinthepresenceofbiotinanti-mouseIgMandFITC-anti-mouseIgA(SouthernBiotechnology).Cytospinswerewashedandsequentiallyincubatedwithstreptavidin,followedbybiotinylated-goatanti-streptavidin(bothVector,Burlingame,CA)andTexasRedstreptavidin(JacksonImmunoResearch),allincubationslastedfor30minandwasheswereperformedinbetweenincubationsandslideswereanalyzed. NumberOfProtocols:2 |
| LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
| IntendedUse | ResearchUseOnly |
| :: | PreservativeStabilisers:0.09%-SodiumAzide 0.2%-BovineSerumAlbumin AntiserumPreparation:AntiseratoratIgG2awereraisedbyrepeatedimmunisationsofgoatswithhighlypurifiedantigen.PurifiedIgGwaspreparedfromwholeserumbyaffinitychromatography. |
| :: | ApproxProteinConc:IgGconcentration1.0mg/ml BufferSolutions:PhosphatebufferedsalinepH7.2 |
