Overview:
GSK3β is a serine threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase (1). GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB.
References:
1. Sutherland C. et al: Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J. 1993 Nov 15;296(Pt1):15-9.
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也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

