Overview:
The Tau family of proteins interacts directly with microtubules to stabilize macromolecular structures. Microtubule association is dependent on the phosphorylation state of Tau proteins (1). Hyperphosphorylated Tau proteins are implicated in Alzheimer's disease (AD) where it is a major component of the offending protein aggregates. Other components of the aggregates include misfolded proteins such as beta-amyloid precursor protein (betaAPP), ubiquitin, and various chaperones and protein kinases. Malfunctioning Tau proteins are associated with microtubule disintegration and collapse of the neuronal transport system (2).
References:
1. Zilka, N., et al. Truncated tau from sporadic Alzheimer's disease suffices to drive neurofibrillary degeneration in vivo. FEBS Lett. 2006; 508: 3582-3588. 2. Rial, A. et al: Calcium Dyshomeostasis in beta-amyloid and tau-bearing skeletal myotubes. J. Biol. Chem., 2004; 279: 3524-53532.
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也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

