Overview:
Acetylation of the histone tail causes chromatin to adopt an “open” conformation, allowing trans factors increased accessibility to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate non-histone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a “closed” chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I (HDACs 1, 2, 3 and 8) proteins are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9 and 10) are related to yeast Hda1-like proteins and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).
References:
1. Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693–703.2. Gregory, P.D. et al. (2001) Exp. Cell Res. 265, 195–202.3. Liu, Y. et al. (2000) Mol. Cell. Biol. 20, 5540–5543.4. Cress, S.D. and Seto, E. (2000) J. Cell. Physiol. 184, 1–16.5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262, 75–83.6. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84–100.7. Viguishin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205–218.
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也就是说只要是兔子产生的抗体，羊抗兔（也叫抗抗体或者2抗）都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中，二抗经常被做了手脚，打上了标记，比如辣根过氧化物酶，经常被用在ELISA，WESTERN等经典的生物学试验中。
