
Overview:
Affinity purified rabbit immunoglobulins were used to raise goat antibodies specific to the rabbit IgG.The goat immune serum is subsequently affinity purified using immunoglobulins immobilized to agarose.The antibodies are then conjugated to horse radish peroxidase (HRP) by reductive amination in a 2:1 molar ratio. The HRP conjugated secondary antibody can recognize the primary immunoglobulin and give a signal in the presence of a chemiluminescent substrate.The signal can be detected using an electronic imager or autoradiography film (1).
References:
1.Nakane PK, Pierce GB: Enzyme-labeled antibodies: preparation and application for localization of antigens, J Histochem Cytochem 1966, 14:929
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也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

