Lyophilized Powder This product is freeze dried. All water molecules have been removed.
Antigen Incl. This antibody is shipped with its antigen FREE of charge!
- Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.

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Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400).
6-10. Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen.
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Expression of EMR1 in rat RBL cellsCell surface detection of EMR1 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red), (1:100). B. Live view of the cells. C. Merged image of panels A and B.
- 1. Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
- 2. McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
- 3. Araç, D. et al. (2012) EMBO J. 31, 1364.
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Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.
Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.
EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.
Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.
Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.
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也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

