INFORMATION: The FOXP3 molecule is a 50–55 kD transcription factor also known as IPEX, JM2, Forkhead box 3, and Scurfin. Defects in this gene can result in lethal autoimmune disease (2). When used with cell surface markers CD4 and CD25(IL-2R), FOXP3 is a useful marker for identifying T regulatory cells. Functionally, FOXP3 is thought to mediate oxidative phosphorylation, enabling Treg to function in a lower oxygen environment (3).
In EIA, clone ANCFX2D7 binds to full length recombinant FOXP3 and to a FOXP3(R1-R3) construct (aa 1–198) but not to a FOXP3(R1) construct (aa 1-70), suggesting that its epitope is within regions 2 and 3 (aa 71–198).
ANCFX2D7 binds to nuclear FOXP3 found constitutively in a 0.5 – 3% sub population of fixed, permeabolized CD4+ peripheral blood lymphoid cells in FACS.
FOXP3 References:
1) G Roncador, A H Banham, et al. (2005) Eur J Immunol 35(6): 1681-1691. PMID: 15902688.
2) S Hori, S Sakaguchi, (2004) Microbes and Infection 6(8): 745-751.
3) U H Beier, W W Hancock, et al. (2016) J Immunol 196(1 suppl 211.3).
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也就是说只要是兔子产生的抗体，羊抗兔（也叫抗抗体或者2抗）都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中，二抗经常被做了手脚，打上了标记，比如辣根过氧化物酶，经常被用在ELISA，WESTERN等经典的生物学试验中。
