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PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.
The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.
NEW: Our hMNC are now also available from HLA-typed donors.
Available formats:
- Cryopreserved: Cryogenic vial containing 25.000.000 viable cells.
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density | 1,000,000 cells per ml |
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Tested Markers | CD31 negative, CD34 negative, CD105 positive, CD146 positive |




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也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

