
Anti-CanineCellpolyclonalantiserumconjugatedtofluoresceinisothiocyanate(FITC).Caprineorigin.Thisconjugateisintendedtoidentifyorconfirmthespeciesoforiginofcellsortissues.Cellsofcanineoriginwillfluorescegreenwhenthisconjugateisappliedpertherecommendedprocedure.Cellsofotherspeciesoriginswillnotfluoresceabovebackgroundlevels.Therecommendedmethodistomixthetestarticlecellswithabenignsubstratecell(monkeykidneyworksfine)inaratioof1:15andplaceonaslideormicroplateculture.FAstainingshouldbedoneat1-3daysofcultureandfixationshouldbe100%acetoneatroomtemperatureforglassslides,oracetone/water(75/25)for20minutesforplastic.
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