Anti-CanineCellpolyclonalantiserumconjugatedtofluoresceinisothiocyanate(FITC).Caprineorigin.Thisconjugateisintendedtoidentifyorconfirmthespeciesoforiginofcellsortissues.Cellsofcanineoriginwillfluorescegreenwhenthisconjugateisappliedpertherecommendedprocedure.Cellsofotherspeciesoriginswillnotfluoresceabovebackgroundlevels.Therecommendedmethodistomixthetestarticlecellswithabenignsubstratecell(monkeykidneyworksfine)inaratioof1:15andplaceonaslideormicroplateculture.FAstainingshouldbedoneat1-3daysofcultureandfixationshouldbe100%acetoneatroomtemperatureforglassslides,oracetone/water(75/25)for20minutesforplastic.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
兔子产生的是一级抗体
羊的为二级抗体
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。

