
Description | Enzyme Immunoassay for the determination of Human ACA-IgG . |
Method Type | Sandwich-Ag |
Sensitivity | 3 μg/mL |
Assay Range | 5~320 μg/mL |
Other Name | |
Principle | This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human ACA-IgG. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Human ACA-IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the substrate solution is added to each well, only those wells that contain Human ACA-IgG , biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACA-IgG. The concentration of Human ACA-IgG in samples can be calculated by comparing the OD of the samples to the standard curve. |
Intended Use | This ELISA kit can be applied to the in vitro quantitative determination of Human ACA-IgG concentrations in serum, plasma and other biological fluids. |
Specificity | This kit recognizes some recombinant and natural Human ACA-IgG. No significant cross-reactivity or interference was observed. |
Sample Type | Serum, plasma and other biological fluids |
Kit Components | Micro ELISA Plate, 8 x 12 well strips Reference Standard, 2 vials Concentrated Biotinylated Detection Ag(100x), 120 µL Concentrated HRP Conjugate(100x), 120 µL Reference Standard and Sample Diluent, 20 mL Biotinylated Detection Ag Diluent, 14 mL HRP Conjugate Diluent, 14 mL Concentrated Wash Buffer (25x), 30 mL Substrate Reagent, 10 mL Stop Solution, 10 mL Plate Sealer, 5 pieces Product Manual Certificate of Analysis |
Other supplies required | Microplate reader with 450nm wavelength filter High-precision transferpettor, EP tubes and disposable pipette tips 37°C Incubator Deionized or distilled water Absorbent paper Loading slot for Wash Buffer |
Storage | All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above. |
Data Sheet E-EL-H0243 (PDF File)
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd
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鼠抗人CD3单克隆抗体针对人的CD3
CD3单克隆抗体,要看其说明书,该抗体是否识别其他生物(如大鼠)的CD3.
本试验阳性结果主要见于下列几种情况:
1.自身免疫性贫血,(IgG)型引起的溶血性贫血,本试验直接反应常呈强阳性,间接反应大多阴性,但亦可阳性。
2.药物诱发的免疫性溶血性贫血①α-甲基多巴型:直接及间接反应均阳性。②青霉素型:直接反应阳性,间接反应阴性,以上二型如以正常红细胞先与有关药物于37℃培育后再加病人血清、间接反应均为阳性。③福阿亭型:(奎宁等药物)抗体通常为IgM,偶有IgG型者,直接反应为阳性,间接反应阴性,但如用IgG抗血清做试剂则结果大部分均为阴性,但如培育时加入新鲜正常人血清(供应补体)则结果为阳性。
3.冷凝集素综合征直接反应阳性,间接反应阴性(试验需在37℃下进行)由于本病红细胞膜附着的是补体C4和C3而不是IgG或IgM,如果用抗IgG或抗IgM抗血清做试验时,则结果阴性,如以抗补体的抗血清做试验则直接反应阳性。
4.新生儿同种免疫溶血病,因Rh血型不合所致溶血病,直接及间接反应均强阳性,持续数周、换血输血后数天内可变为阴性,由于“ABO”血型不合引起的溶血病,结果常为阴性或弱阳性。
5.红细胞血型不合引起的输血反应,ABO或Rh血型不合输血,供者的红细胞被受者的血型抗体致敏,在供者被致敏的红细胞完全破坏以前,直接反应阳性,Rh阴性者如过去不曾接受过Rh阳性者的血或曾妊娠胎儿为Rh阳性者,间接反应阳性,如无上述接触,第一次输血后(Rh阳性的血),数天之内间接反应也会变为阳性。
6.其它在传染性单核细胞增多症、SLE、恶性淋巴瘤、慢性淋巴细胞白血病、癌肿、铅中毒、结节性动脉周围炎、EVan氏综合征等,病人直接反应亦可阳性,阵发性寒冷性血红蛋白尿症患者中,急性发作后用抗补体血清做试验直接反应常为阳性。展开

