Cell Line Information:Cell Line Name: A-498 (ATCC® HTB-44™)Organism: Homo sapiens, human Tissue: kidney Morphology: epithelial Culture Properties: adherent Biosafety Level: 1 Disease: carcinoma Age: 52 years Gender: female Contributor: S. AaronsonSupplier: Developmental Therapeutics Program.National Cancer Institute,Frederick,MD 21701A-498 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.The Transfection Reagent:A-498 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting A-498 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for A-498 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into A-498 cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate format
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商,公司的研发宗旨是让生命科学研究变得更简单,快速,容易!该公司主要产品之一为新一代转染试剂Avalanche 系列产品,该产品集合了组合化学,分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成,包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。
EZBiosystems特约代理EZBiosystems品牌名称:EZBiosystemsEZBiosystems品牌logo:EZBiosystems品牌官网:http://ezbiosystems.com/EZBiosystems品牌链接:http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍:EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品:品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml
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抗人球蛋白试验,又称Coombs’试验,是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性,Coombs’试验阴性有时并不能排除AIHA

