Cell Information:

Airway epithelial cells are targets in asthma, viral infection and fibrotic lung disease. Airway epithelial cells include human Bronchial, Tracheal, and Small Airway Epithelial Cells. These cells have been used to study the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells.

Applications:

• Asthma
• COPD
• Airborne viruses
• Allergies
• Inhalation of nanoparticles
• Cancer
• Cystic Fibrosis
• DoD studies

The Transfection Reagent:

Transfection of primary cells, such as Human Airway Epithelial Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Airway Epithelial Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for Human Airway Epithelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent? series?

References

1. Oniki K, Nohara H, Nakashima R, Obata Y, Muto N, Sakamoto Y, Ueno-Shuto K, Imafuku T, Ishima Y, Watanabe H, Maruyama T, Otake K, Ogata Y, Suico MA, Kai H, Shuto T, Saruwatari J.The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties. Sci Rep. 2020 Apr 6;10(1):5973. doi: 10.1038/s41598-020-62872-5. PubMed PMID: 32249844; PubMed Central PMCID: PMC7136289.
2. Zhou Y, Li P, Goodwin AJ, Cook JA, Halushka PV, et al.Exosomes from endothelial progenitor cells improve outcomes of the lipopolysaccharide-induced acute lung injury. Crit Care. 2019 Feb 13;23(1):44. PubMed PMID: 30760290; PubMed Central PMCID: PMC6373158.

Features:

• Specifically optimized to deliver nucleic acids into Human Airway Epithelial Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商，公司的研发宗旨是让生命科学研究变得更简单，快速，容易！该公司主要产品之一为新一代转染试剂Avalanche 系列产品，该产品集合了组合化学，分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成，包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。

EZBiosystems特约代理EZBiosystems品牌名称：EZBiosystemsEZBiosystems品牌logo：EZBiosystems品牌官网：http://ezbiosystems.com/EZBiosystems品牌链接：http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍：EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品：品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml