Cell Information:

Human Cardiac Fibroblasts (HCF, NHCF-A and -V) are derived from normal human heart tissues (atrium or ventricle).Aortic Adventitial Fibroblasts (AoAF) (isolated from the tunica external of the ascending or descending aorta) areCardiac Related fibroblasts . Human Cardiac fibroblasts or Cardiac Related Fibroblasts have been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function. Expression of MyoD can induce myogenesis of cardiac fibroblasts. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production.

Applications:

• Vascular diseases
• Neointimal formation
• Vascular remodeling
• Atherosclerosis
• Hypertension
• Myocardial repair
• Extracellular maintenance during cardiac cell / tissue growth
• Cardiac Fibrosis Paracrine signalling
• Toxicology
• Gene expression

The Transfection Reagent:

Transfection of primary cells, such as Human Cardiac Fibroblasts or Cardiac Related Fibroblasts, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Cardiac fibroblasts and Cardiac Related Fibroblasts. No other transfection reagents can match the efficiency, convenience, and gentleness of The Reagent for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Human Cardiac fibroblasts and Cardiac Related Fibroblasts.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Developed and manufactured by EZ Biosystems

EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商，公司的研发宗旨是让生命科学研究变得更简单，快速，容易！该公司主要产品之一为新一代转染试剂Avalanche 系列产品，该产品集合了组合化学，分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成，包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。

EZBiosystems特约代理EZBiosystems品牌名称：EZBiosystemsEZBiosystems品牌logo：EZBiosystems品牌官网：http://ezbiosystems.com/EZBiosystems品牌链接：http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍：EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品：品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml