Cell Information:

Human Fibroblast-like synoviocytes (HFLS) represent a specialised cell type located inside joints in the synovium. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations

Human Fibroblast-Like Synoviocytes (HFLS) are usually isolated from normal synovial tissues. These cells can be used as a good in vitro model for studyingthe pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis.

The Transfection Reagent:

Transfection of primary cells, such as HFLS, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HFLS. The proprietary formulation oflipids and polymers ensures the highest possible transfection efficiencies and viabilities for HFLS. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent for transfection on HFLS. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商，公司的研发宗旨是让生命科学研究变得更简单，快速，容易！该公司主要产品之一为新一代转染试剂Avalanche 系列产品，该产品集合了组合化学，分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成，包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。

EZBiosystems特约代理EZBiosystems品牌名称：EZBiosystemsEZBiosystems品牌logo：EZBiosystems品牌官网：http://ezbiosystems.com/EZBiosystems品牌链接：http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍：EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品：品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml